FLECT (Fluorescence Emission Computed Tomography) reconstructs the 3D tomographic images from the photon emission data acquired 360° around the animal subject (projection images) using a state-of-the-art 3D reconstruction engine based on an accurate model of light propagation in biological tissue. The result is the most accurate optical quantitation available.

The FLECT laser excites the fluorophore inside the animal subject, causing the fluorophore to emit light at a different wavelength, or fluorescence. The fluorescence is recorded by an array of detectors around the animal subject. Then the gantry rotates, changing the position of the laser and detectors, for acquiring data at a different angle. This process is repeated again and again until the subject has been imaged at all angles covering 360° for each individual slice. The animal bed is then moved to the next slice position so that the animal subject can be imaged along its entire body.